Toxicity and Bioaccumulation of Copper in Phaeodactylum tricornutum Under Different Macronutrient Conditions

In this study, we investigated cell physiological and biochemical responses to copper (Cu) accumulation in the marine diatom Phaeodactylum tricornutum under different nitrogen (N) phosphorus (P) conditions. Cells with sufficient N P (+N+P) showed highest total Cu concentrations displayed a higher metal-tolerance ability. +N+P treatments, 36.5% of dissolved was adsorbed on frustules (cell walls), 54.9% occluded organelles, 8.6% soluble fraction. comparison, cells and/or shortages (+N−P, −N+P, −N−P) 10.8–13.1% onto their walls, 74.5–80% 9.2–12.4% The 2+ influx wall faster due metal ligands walls up-regulated metal-related transporters. A significant increase protein content occurred shortages, which accompanied by inhibition antioxidative enzymatic system membrane damage. Our findings indicate that play an important role both adsorption surface) uptake (intracellular), they provide multifaceted evidence acquisition mechanism P. macronutrient